This study focuses on the automated extraction of small volumes of urine samples (< 500 μL) using disposable pipette extraction (DPX) for the comprehensive screening of pain management drugs by LC-MS/MS.
Using a GERSTEL MPS autosampler, DPX extractions of hydrolyzed urine were performed, using a reversed phase (DPX-RP-S) sorbent. The resulting eluents were diluted and analyzed by LC-MS/MS allowing rapid, “just-in-time” sample preparation for high throughput screenings, averaging an injection to injection cycle time of 7 min/sample.
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