New AppNote: Benzodiazepines in urine using DPX-LC/MS/MS

Automated Disposable Pipette Extraction DPX coupled to LC/MS/MS eliminates the need for derivatization when determining benzodiazepines in urine.



Analysis of benzodiazepines using GC/MS necessitates the use of derivatization, which has been shown to be a limitation for some of the benzodiazepines. The analysis of automated DPX extracted benzodiazepines using LC/MS/MS was chosen in an attempt to eliminate the need for derivatization.
Previous data described in Agilent Application Notes show the use of an Agilent 6410 LC/MS/MS instrument to be a highly sensitive procedure for the analysis of benzodiazepines, with limits of quantitation of 5 ng/mL.

This study focuses on the automated extraction of small sample volumes coupled to LC/MS/MS in order to provide high throughput analysis for drugs of abuse such as benzodiazepines. Using a GERSTEL MPS 2 autosampler, the DPX extractions were performed using a mixed-mode cation exchange (DPX-CX) sorbent. The resulting eluents from the automated DPX extractions were then introduced into an Agilent 6410 LC/MS/MS instrument.

Analysis of Drugs of Abuse using Automated Disposable Pipette Extraction and LC/MS/MS