30. Oktober 2015
Metabolomics AppNote 2/3: Automated Lipid Fractionation using SPE
Automated sample preparation including SPE fractionation, evaporation and reconstitution for LC/QTOF analysis of different Lipid classes from plant material
Abstract:
In metabolomics studies, large sample sets have to be
analyzed to allow statistical differentiation of sample types.
Obviously, repeatability of the whole analytical work
flow, including sample preparation, sample introduction,
separation and detection, is hereby of the utmost importance.
In this respect, automation of the sample preparation is very
useful in order to reduce the analytical variability.
In a series of articles, we describe the use of the Gerstel
MPS WorkStation for automated sample preparation applied
to metabolomics studies. In a first part, an automated
ultrasonic assisted extraction and filtration method was
discussed. In this second part, an automated fractionation of
lipid classes using solid phase extraction (SPE) is presented.
The SPE fractions are concentrated using an mVAP
evaporation station and re-dissolved in small amounts of
solvent, followed by LC-QTOF analysis.
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AppNote-2015-01 Automated Sample Preparation for Metabolomics Studies Using the Gerstel MPS Dual Head WorkStation Part 2: Automated Lipid Fractionation Using Solid Phase Extraction |